Journal: Frontiers in Microbiology

Article Title: Functional Gene Identification and Corresponding Tolerant Mechanism of High Furfural-Tolerant Zymomonas mobilis Strain F211

doi: 10.3389/fmicb.2021.736583

Figure Lengend Snippet: Forces in Zymomonas mobilis flocculation. (A) Flocculent microscopic image of recombinant strains. (B) Flocculation rate. (C) Flocculation phenotype.

Article Snippet: The wild-type strain Z. mobilis CP4 was purchased from the China Center for Type Culture Collection (CCTTCC; Accession NO.CICC10232).

Techniques: Flocculation, Recombinant

Journal: Frontiers in Microbiology

Article Title: Functional Gene Identification and Corresponding Tolerant Mechanism of High Furfural-Tolerant Zymomonas mobilis Strain F211

doi: 10.3389/fmicb.2021.736583

Figure Lengend Snippet: Potential furfural tolerance mechanism of Z. mobilis .

Article Snippet: The wild-type strain Z. mobilis CP4 was purchased from the China Center for Type Culture Collection (CCTTCC; Accession NO.CICC10232).

Techniques:

Journal: Bioengineered

Article Title: Engineering Saccharomyces cerevisiae for improvement in ethanol tolerance by accumulation of trehalose

doi: 10.1080/21655979.2016.1207019

Figure Lengend Snippet: Strains used in this study.

Article Snippet: S. cerevisiae (BCRC 21685) was purchased from Bioresource Collection and Research Center, Food Industry Research and Development Institution, Hsinchu, Taiwan.

Techniques: Over Expression, Plasmid Preparation

Journal: Bioengineered

Article Title: Engineering Saccharomyces cerevisiae for improvement in ethanol tolerance by accumulation of trehalose

doi: 10.1080/21655979.2016.1207019

Figure Lengend Snippet: Construction of deletion cassette with long flanking homology regions by overlapping PCR. In the first round of PCR, the upstream sequence of the nth1 gene, the kanr marker gene and the downstream sequence of the nth1 gene were amplified in 3 different PCR reaction tubes. S. cerevisiae genomic DNA was used as template for amplification of 0.26 kb 5′ UP nth1 region and 0.4 kb 3′ DOWN nth1 region by using primers L1, L2, and L3, L4, respectively. The pFA6a-kanMX6 vector was used as a template for amplification of kanMX region by using primers kanMX-F and kanMX-R. In the second round of PCR, the 5′ UP nth1 region and kanMX region were fused together by using primers L1 and kanMX-R. In the third round of PCR, the 3′ DOWN nth1 region and second round PCR product (5′ UP nth1 region-kanMX region) were fused together by using primers L1 and L4. The third round PCR product (5′ UP nth1 region- kanMX region - 3′ DOWN nth1 region) was cloned into pGEMT vector for construction of the disruption cassette. After transformation of the disruption cassette into S. cerevisiae, homologous recombination takes place with the deletion of target gene.

Article Snippet: S. cerevisiae (BCRC 21685) was purchased from Bioresource Collection and Research Center, Food Industry Research and Development Institution, Hsinchu, Taiwan.

Techniques: Sequencing, Marker, Amplification, Plasmid Preparation, Clone Assay, Transformation Assay, Homologous Recombination

Journal: Scientific Reports

Article Title: High mannose-specific lectin Msl mediates key interactions of the vaginal Lactobacillus plantarum isolate CMPG5300

doi: 10.1038/srep37339

Figure Lengend Snippet: Strains and plasmids used in this study.

Article Snippet: WCFS1 , Sequenced wild-type strain; single colony isolate of NCIMB 8826 from human saliva , .

Techniques: Isolation, Mutagenesis, Expressing, Bacteria

Journal: PLoS ONE

Article Title: The Identification of Congeners and Aliens by Drosophila Larvae

doi: 10.1371/journal.pone.0136363

Figure Lengend Snippet: Larval locomotion of D . melanogaster (the Oregon R-c, Canton-Special, Til-Til, Trana, vestigial , Orco , Syn 97CS and rut strains), D . pavani (La Florida strain), D . gaucha (the Buenos Aires strain), and the pavani female x gaucha male and gaucha female x pavani male hybrids. Locomotion was measured in four conditions: (i) on agar (agar aroma, controls), (ii) agar plus synthetic food aroma, (iii) on agar plus conspecific odors, and (iv) on agar plus alien food aroma. For D . melanogaster larvae, D . pavani food was used as alien food. To D . pavani , D . gaucha and the hybrid larvae, alien food means D . melanogaster (Oregon R-c) food. Locomotion of N = 50 third instar larvae of each strain and species was measured by 2 min (see Materials and Methods ).

Article Snippet: The wild type Trana strain of D . melanogaster was established with adults that emerged from grape ( Vitis vinifera , País Variety); no other Drosophila species emerged from the decaying fruit.

Techniques:

Journal: PLoS ONE

Article Title: The Identification of Congeners and Aliens by Drosophila Larvae

doi: 10.1371/journal.pone.0136363

Figure Lengend Snippet: (A–H), response to sterile food aroma and food processed by Oregon R-c larvae ( D . melanogaster ). (I–P), response to sterile food aroma and food worked by La Florida larvae ( D . pavani ). Navigation toward sterile food, congeners and alien odors is shown as percentage of larvae ± SE arriving at the papers. Black circle ( A–P ), filter paper moistened in sterile food. White triangle ( A–H ), filter paper moistened in food used by Oregon R-c larvae ( D . melanogaster ) or La Florida larvae ( D . pavani ) ( I–P ). When standard errors are not shown is because they are too small.

Article Snippet: The wild type Trana strain of D . melanogaster was established with adults that emerged from grape ( Vitis vinifera , País Variety); no other Drosophila species emerged from the decaying fruit.

Techniques: Sterility

Journal: PLoS ONE

Article Title: The Identification of Congeners and Aliens by Drosophila Larvae

doi: 10.1371/journal.pone.0136363

Figure Lengend Snippet: ( A—D ), percentage of larvae on the paper impregnated in sterile food (black circles) and on the paper moistened in food used by larvae of the species or the respective hybrid (white triangles). ( E–H ), percentage of larvae on papers impregnated in sterile food and Oregon R-c ( D . melanogaster ) food. See also for further details.

Article Snippet: The wild type Trana strain of D . melanogaster was established with adults that emerged from grape ( Vitis vinifera , País Variety); no other Drosophila species emerged from the decaying fruit.

Techniques: Sterility

Journal: PLoS ONE

Article Title: The Identification of Congeners and Aliens by Drosophila Larvae

doi: 10.1371/journal.pone.0136363

Figure Lengend Snippet: White triangles, percentage of larvae on the paper moistened in food used by the species or the respective hybrid. Black circles, percentage of larvae on the paper impregnated in food used by Oregon R-c larvae ( D . melanogaster ). ( A ) D . pavani larvae; ( B ) D . gaucha larvae; ( C ) pavani female x gaucha male hybrid larvae; ( D ) gaucha female x pavani male hybrid larvae.

Article Snippet: The wild type Trana strain of D . melanogaster was established with adults that emerged from grape ( Vitis vinifera , País Variety); no other Drosophila species emerged from the decaying fruit.

Techniques: